Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 156.411, 156.411, 116.205 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.243 - 3.310 |
| Rwork | 0.261 |
| R-free | 0.30520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zby |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.626 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.253 | 3.400 |
| High resolution limit [Å] | 3.310 | 3.310 |
| Rmerge | 0.091 | 3.900 |
| Rpim | 0.770 | |
| Number of reflections | 22105 | 1538 |
| <I/σ(I)> | 0.018 | 0.5 |
| Completeness [%] | 100.0 | |
| Redundancy | 25.6 | |
| CC(1/2) | 1.000 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | mixing 0.2 uL of the 18 mg/mL complex with 0.1 uL of the crystallization buffer containing 0.2 M Sodium acetate trihydrate, 0.1 M MES pH 6.0, 20 % w/v PEG 8000. |
