6ZH1

Crystal structure of complex between FH19-20 and FhbA protein from Borrelia hermsii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-29
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.87290
Spacegroup name	P 21 21 21
Unit cell lengths	43.273, 60.327, 145.681
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.880 - 2.200
R-factor	0.193
Rwork	0.190
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g7i
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	autoPROC (10.10.5)
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.828	2.237
High resolution limit [Å]	2.200	2.200
Rmerge	0.166	2.436
Rmeas	0.172	2.529
Rpim	0.047	0.673
Number of reflections	20180	993
<I/σ(I)>	12.531	1
Completeness [%]	100.0	99
Redundancy	13.1	13.78
CC(1/2)	0.999	0.513

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293.15	0.1 M MES pH6.7, 0.2 M Ammonium Sulfate, 20% (v/v) PEG 4000