6ZGT
Crystal structure of a MFS transporter with bound 2-naphthoic acid at 2.39 Angstroem resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 102.534, 199.623, 61.934 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.962 - 2.230 |
R-factor | 0.2164 |
Rwork | 0.215 |
R-free | 0.23990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6g9x |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.1) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.14_3228) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.660 | 49.660 | 2.350 |
High resolution limit [Å] | 2.230 | 7.050 | 2.230 |
Rmerge | 0.139 | 0.094 | |
Rmeas | 0.140 | 0.095 | 75.194 |
Rpim | 0.019 | 0.013 | 10.243 |
Total number of observations | 3733279 | ||
Number of reflections | 62970 | 2199 | 9044 |
<I/σ(I)> | 28.9 | ||
Completeness [%] | 100.0 | 99.7 | 99.9 |
Redundancy | 59.3 | 47.7 | 52 |
CC(1/2) | 0.998 | 0.997 | 0.251 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291.15 | zinc bromide, HEPES, jeffamine ED-2003 |