6ZGN
Crystal structure of VirB8-like OrfG central domain of Streptococcus thermophilus ICESt3; a putative assembly factor of a gram positive conjugative Type IV secretion system.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-16 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 50.162, 50.162, 120.883 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.331 - 1.750 |
| Rwork | 0.200 |
| R-free | 0.20810 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.635 |
| Data reduction software | XDS (20190315) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1-3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.331 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.027 | 0.499 |
| Rmeas | 0.028 | 0.511 |
| Rpim | 0.006 | 0.105 |
| Number of reflections | 16372 | 869 |
| <I/σ(I)> | 67.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 25.1 | 23.8 |
| CC(1/2) | 1.000 | 0.975 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | Ammonium sulfate; 2M Tris pH 8.5; 100 mM |
