6ZGN

Crystal structure of VirB8-like OrfG central domain of Streptococcus thermophilus ICESt3; a putative assembly factor of a gram positive conjugative Type IV secretion system.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980
Spacegroup name	P 41 21 2
Unit cell lengths	50.162, 50.162, 120.883
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.331 - 1.750
Rwork	0.200
R-free	0.20810
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.635
Data reduction software	XDS (20190315)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1-3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.331	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.027	0.499
Rmeas	0.028	0.511
Rpim	0.006	0.105
Number of reflections	16372	869
<I/σ(I)>	67.5
Completeness [%]	100.0	100
Redundancy	25.1	23.8
CC(1/2)	1.000	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	Ammonium sulfate; 2M Tris pH 8.5; 100 mM