6ZGI
Furin Cleaved Spike Protein of SARS-CoV-2 in Closed Conformation
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Quantum LS |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.9 |
| Software | crYOLO,cryoSPARC,RELION,EPU,CTFFIND,Coot,PHENIX |
| Symmetry | C3 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
