6ZFG
14-3-3 zeta chimera with 18E6 and fusicoccin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-17 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.230, 76.100, 88.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.400 - 1.850 |
R-factor | 0.1921 |
Rwork | 0.191 |
R-free | 0.21920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o02 |
RMSD bond length | 0.014 |
RMSD bond angle | 0.949 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.400 | 1.900 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmeas | 0.163 | 2.160 |
Number of reflections | 41927 | 3038 |
<I/σ(I)> | 12.36 | 1.4 |
Completeness [%] | 97.1 | 96.5 |
Redundancy | 13.3 | 13.6 |
CC(1/2) | 0.998 | 0.584 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 19% PEG4000, 0.1M cacodylate, 20%glycerol as a cryoprotectant |