6ZEQ

Aspergillus oryzae Leucine Aminopeptidase A mature enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 63 2 2
Unit cell lengths	153.920, 153.920, 88.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	73.810 - 1.970
R-factor	0.1664
Rwork	0.165
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rtq
Data reduction software	xia2
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.810	2.041
High resolution limit [Å]	1.970	1.970
Rmerge	0.220	2.178
Number of reflections	43994	4297
<I/σ(I)>	10.35	1.18
Completeness [%]	100.0
Redundancy	13.1
CC(1/2)		0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M Citrate, pH = 5.0, 3.2 M Ammonium sulphate