6ZEP
Flavourzyme Leucine Aminopeptidase A proenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-03 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.430, 94.390, 99.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.611 - 1.610 |
R-factor | 0.1537 |
Rwork | 0.153 |
R-free | 0.17390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rtq |
RMSD bond length | 0.007 |
RMSD bond angle | 0.930 |
Data reduction software | xia2 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.611 | 1.668 |
High resolution limit [Å] | 1.610 | 1.610 |
Rmerge | 0.073 | 0.891 |
Rmeas | 0.083 | |
Number of reflections | 53338 | 5269 |
<I/σ(I)> | 11.36 | 1.59 |
Completeness [%] | 99.5 | |
Redundancy | 4.3 | |
CC(1/2) | 0.550 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris, pH = 5.5, 0.2 M NaCl, 25% PEG 3,350 |