Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.910, 80.759, 83.783 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.953 - 1.650 |
Rwork | 0.162 |
R-free | 0.18240 |
Structure solution method | SIRAS |
RMSD bond length | 0.005 |
RMSD bond angle | 1.283 |
Data reduction software | Aimless (1.0.2) |
Data scaling software | Aimless (1.0.2) |
Phasing software | AutoSol (1.17.1) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.950 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.044 | |
Rmeas | 0.048 | |
Rpim | 0.018 | |
Number of reflections | 62534 | 2937 |
<I/σ(I)> | 21.8 | |
Completeness [%] | 99.8 | 96.9 |
Redundancy | 12.4 | 9.1 |
CC(1/2) | 0.999 | 0.594 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291.15 | 100 mM Hepes/NaOH pH 7.0, 200 mM L-proline and 12% (w/v) PEG3350 | |
1 | VAPOR DIFFUSION | 291.15 | 100 mM Hepes/NaOH pH 7.0, 200 mM L-proline and 12% (w/v) PEG3350 |