6ZDU

Structure of telomerase from Candida albicans in complexe with TWJ fragment of telomeric RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-08
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.980109
Spacegroup name	P 21 21 2
Unit cell lengths	149.884, 245.836, 72.471
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.900 - 3.450
R-factor	0.2503
Rwork	0.248
R-free	0.30080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zdp
RMSD bond length	0.002
RMSD bond angle	0.514
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.900	3.788
High resolution limit [Å]	3.450	3.450
Rmerge	0.114	1.824
Rmeas	0.123	1.940
Rpim	0.043	0.735
Number of reflections	23166	1158
<I/σ(I)>	14.1	1.5
Completeness [%]	92.1	53.9
Redundancy	13.4	13.1
CC(1/2)	0.999	0.617

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	291	0.1 M imidazole 0.1 M MES monohydrate, pH 6.5 0.02 M sodium formate 0.02 M ammonium acetate 0.02 M sodium citrate tribasic dihydrate 0.02 M sodium potassium tartrate tetrahydrate 0.02 M sodium oxamate 12% glycerol and 7 % PEG4000