6ZDU
Structure of telomerase from Candida albicans in complexe with TWJ fragment of telomeric RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-08 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.980109 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 149.884, 245.836, 72.471 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.900 - 3.450 |
R-factor | 0.2503 |
Rwork | 0.248 |
R-free | 0.30080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6zdp |
RMSD bond length | 0.002 |
RMSD bond angle | 0.514 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.900 | 3.788 |
High resolution limit [Å] | 3.450 | 3.450 |
Rmerge | 0.114 | 1.824 |
Rmeas | 0.123 | 1.940 |
Rpim | 0.043 | 0.735 |
Number of reflections | 23166 | 1158 |
<I/σ(I)> | 14.1 | 1.5 |
Completeness [%] | 92.1 | 53.9 |
Redundancy | 13.4 | 13.1 |
CC(1/2) | 0.999 | 0.617 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 291 | 0.1 M imidazole 0.1 M MES monohydrate, pH 6.5 0.02 M sodium formate 0.02 M ammonium acetate 0.02 M sodium citrate tribasic dihydrate 0.02 M sodium potassium tartrate tetrahydrate 0.02 M sodium oxamate 12% glycerol and 7 % PEG4000 |