6ZDQ

Structure of telomerase from Candida albicans in complexe with TWJ fragment of telomeric RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978520
Spacegroup name	P 43 21 2
Unit cell lengths	74.156, 74.156, 451.418
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.450 - 2.980
R-factor	0.2411
Rwork	0.238
R-free	0.29200
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.470
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.450	3.091
High resolution limit [Å]	2.980	2.984
Rmerge	0.069	1.030
Rmeas	0.072	1.074
Rpim	0.021	0.303
Number of reflections	25498	2662
<I/σ(I)>	26.31	2.68
Completeness [%]	93.6	99.44
Redundancy	12.6	12.4
CC(1/2)	0.997	0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	0.2 M sodium citrate tribasic dihydrate 0.1 M Bis-Tris propane (pH 7.5) 17% PEG2000-MME