6ZDP

Structure of telomerase from Candida Tropicalis in complexe with TWJ fragment of telomeric RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978530
Spacegroup name	C 2 2 21
Unit cell lengths	95.095, 201.729, 138.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.700 - 2.850
R-factor	0.2227
Rwork	0.220
R-free	0.27820
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.434
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.865	2.952
High resolution limit [Å]	2.850	2.850
Rmerge	0.145	1.831
Rmeas	0.157	1.980
Rpim	0.059	0.750
Number of reflections	41766	805
<I/σ(I)>	13.4	1.6
Completeness [%]	93.6
Redundancy	13.2
CC(1/2)	0.999	0.616

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.1	298	0.2M sodium citrate tribasic dihydrate 0.1M Bis-Tris propane 13% PEG 2000 MME