6ZD1
Structure of apo telomerase from Candida Tropicalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978530 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 65.798, 65.798, 387.948 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.780 - 2.470 |
R-factor | 0.248 |
Rwork | 0.246 |
R-free | 0.28410 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.718 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.780 | 2.555 |
High resolution limit [Å] | 2.467 | 2.467 |
Rmerge | 0.150 | 1.652 |
Rmeas | 0.163 | 1.804 |
Rpim | 0.062 | 0.507 |
Number of reflections | 54095 | 996 |
<I/σ(I)> | 10.47 | 1.4 |
Completeness [%] | 85.1 | 45.9 |
Redundancy | 6.8 | 12.6 |
CC(1/2) | 0.991 | 0.543 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.1 | 298 | 0.2 M sodium citrate tribasic dihydrate 0.1 M Bis-Tris propane (pH 6.1) 15% PEG3350 |