6ZCO

Crystal Structure of C-terminal Dimerization Domain of Nucleocapsid Phosphoprotein from SARS-CoV-2, crystal form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	I 41
Unit cell lengths	88.203, 88.203, 42.763
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.361
R-factor	0.1517
Rwork	0.149
R-free	0.19620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yun
RMSD bond length	0.016
RMSD bond angle	1.645
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.096	44.096	1.430
High resolution limit [Å]	1.360	4.300	1.361
Rmerge		0.018	1.093
Rmeas	0.047	0.020	1.446
Rpim	0.016	0.006	0.698
Number of reflections	34588	1178	4414
<I/σ(I)>	20.2	32.6	0.7
Completeness [%]	97.8	99.9	86.6
Redundancy	7.6	8.9	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		277	tba