6ZB8

Exo-beta-1,3-glucanase from moose rumen microbiome, active site mutant E167Q/E295Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.826561
Spacegroup name	P 1 21 1
Unit cell lengths	55.630, 87.790, 70.860
Unit cell angles	90.00, 100.96, 90.00

Refinement procedure

Resolution	29.350 - 1.350
R-factor	0.1529
Rwork	0.153
R-free	0.16250
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	Wild-type structure solved by MR-SAD
RMSD bond length	0.008
RMSD bond angle	1.007
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.350	1.400
High resolution limit [Å]	1.350	1.350
Number of reflections	139214	10636
<I/σ(I)>	13.2	1.1
Completeness [%]	95.2	70.8
Redundancy	6.6	5
CC(1/2)	0.997	0.373

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1M TrisHCl pH 8.5, 0.2M MgCl, 30% PEG 4000