6ZAZ

Fructo-oligosaccharide transporter BT 1762-63

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	C 2 2 21
Unit cell lengths	119.566, 237.835, 170.505
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	106.826 - 2.690
R-factor	0.2117
Rwork	0.208
R-free	0.27530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t3r
RMSD bond length	0.008
RMSD bond angle	1.105
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	170.500	2.740
High resolution limit [Å]	2.690	2.690
Rpim	0.085	0.610
Number of reflections	67688	3382
<I/σ(I)>	6	1.1
Completeness [%]	100.0
Redundancy	7.2
CC(1/2)	0.990	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.15 M sodium formate 0.1 M Hepes pH 7-7.5 16-20% PEG3350