6ZAB

Structure of the transcriptional repressor Atu1419 (VanR) from agrobacterium fabrum in complex a palindromic DNA (P6422 space group)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 64 2 2
Unit cell lengths	179.180, 179.180, 96.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.610 - 2.800
R-factor	0.189
Rwork	0.188
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z74
RMSD bond length	0.009
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.610	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.137
Number of reflections	23104	1635
<I/σ(I)>	14.3
Completeness [%]	99.7
Redundancy	19.5
CC(1/2)	0.999	0.388

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	292	Terbutanol, Na-citrate, MgCl2