6Z9Y

Copper transporter OprC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.40889
Spacegroup name	P 1 21 1
Unit cell lengths	67.341, 197.668, 171.749
Unit cell angles	90.00, 88.75, 90.00

Refinement procedure

Resolution	85.850 - 2.950
R-factor	0.2451
Rwork	0.242
R-free	0.30220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fok
RMSD bond length	0.009
RMSD bond angle	1.163
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	197.670	3.000
High resolution limit [Å]	2.950	2.950
Rpim	0.081
Number of reflections	94129	9369
<I/σ(I)>	5.56
Completeness [%]	100.0
Redundancy	6.6
CC(1/2)		0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.34 M Ammonium sulfate 0.1 M Sodium citrate 12 -16 % w/v PEG 4000 2 mM CuSO4