6Z9M

Pseudoatomic model of the pre-fusion conformation of glycoprotein B of Herpes simplex virus 1

Replaces:  5FZ2

EM specimen

Vitrification	Yes
Instrument	HOMEMADE PLUNGER

EM Data Collection

Microscope	FEI POLARA 300
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2
Energy Filter	GIF Quantum LS
Detector	GATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

Method	SUBTOMOGRAM AVERAGING
Resolution (Å)	9.1
Software	3dmod,PEET,MDFF,SerialEM,NOVACTF,CTFPHASEFLIP,UCSF Chimera
Symmetry	C3
CTF correction	PHASE FLIPPING ONLY