6Z9C
Structure of human POLDIP2, a multifaceted adaptor protein in metabolism and genome stability
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS MICROFOCUS |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2019-02-22 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 62 |
| Unit cell lengths | 120.138, 120.138, 49.516 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.880 - 2.800 |
| R-factor | 0.2199 |
| Rwork | 0.216 |
| R-free | 0.28810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1VBV 5hdw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.608 |
| Data reduction software | PROTEUM2 |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 104.042 | 23.869 | 2.830 |
| High resolution limit [Å] | 2.687 | 8.500 | 2.690 |
| Rmerge | 0.033 | 2.049 | |
| Rmeas | 0.159 | 0.035 | 2.397 |
| Rpim | 0.058 | 0.012 | 1.216 |
| Total number of observations | 2998 | 6052 | |
| Number of reflections | 11561 | 372 | 1656 |
| <I/σ(I)> | 13.2 | 57.1 | 0.7 |
| Completeness [%] | 99.6 | 94.3 | 98.7 |
| Redundancy | 7.1 | 8.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Calcium acetate hydrate 0.1M Sodium cacodylate pH 6.5 40% (v/v) PEG 300 |
