6Z8Y
Copper transporter OprC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.40889 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 67.032, 170.742, 198.043 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.030 - 2.780 |
R-factor | 0.2131 |
Rwork | 0.210 |
R-free | 0.26620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fok |
RMSD bond length | 0.009 |
RMSD bond angle | 1.116 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 198.040 | 2.830 |
High resolution limit [Å] | 2.780 | 2.780 |
Rpim | 0.078 | |
Number of reflections | 58146 | 5698 |
<I/σ(I)> | 5.52 | |
Completeness [%] | 100.0 | |
Redundancy | 12.66 | |
CC(1/2) | 0.400 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.34 M Ammonium sulfate 0.1 M Sodium citrate 12 -16 % w/v PEG 4000 |