6Z8S

Copper transporter OprC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-09
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.351326
Spacegroup name	C 2 2 21
Unit cell lengths	156.100, 195.690, 166.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.020 - 2.370
R-factor	0.2203
Rwork	0.219
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fok
RMSD bond length	0.008
RMSD bond angle	0.988
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.850	2.410
High resolution limit [Å]	2.370	2.370
Rpim	0.044
Number of reflections	103185	10239
<I/σ(I)>	12.46
Completeness [%]	100.0
Redundancy	13.27
CC(1/2)		0.315

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M NaCl 0.15 M NH4SO4 0.1 M MES 18-22% PEG1000 3 mM CuSO4