6Z8R

Copper transporter OprC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-09
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.351326
Spacegroup name	C 2 2 21
Unit cell lengths	155.030, 196.860, 165.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.440 - 2.380
R-factor	0.2186
Rwork	0.217
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fok
RMSD bond length	0.009
RMSD bond angle	0.992
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.090	2.420
High resolution limit [Å]	2.380	2.380
Rpim	0.044
Number of reflections	101148	10029
<I/σ(I)>	11.71
Completeness [%]	100.0
Redundancy	13.57
CC(1/2)		0.321

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M NaCl 0.15 M NH4SO4 0.1 M MES 18-22% PEG1000