6Z8Q
Copper transporter OprC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.54977 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 156.200, 195.290, 167.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 97.650 - 2.710 |
| R-factor | 0.2174 |
| Rwork | 0.216 |
| R-free | 0.25390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fok |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.047 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 121.980 | 2.760 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Rpim | 0.028 | |
| Number of reflections | 69478 | 6843 |
| <I/σ(I)> | 20.12 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 35.5 | |
| CC(1/2) | 0.653 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M NaCl 0.15 M NH4SO4 0.1 M MES 18-22% PEG1000 2 mM AgNO3 |
