6Z8Q

Copper transporter OprC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.54977
Spacegroup name	C 2 2 21
Unit cell lengths	156.200, 195.290, 167.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	97.650 - 2.710
R-factor	0.2174
Rwork	0.216
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fok
RMSD bond length	0.008
RMSD bond angle	1.047
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	121.980	2.760
High resolution limit [Å]	2.710	2.710
Rpim	0.028
Number of reflections	69478	6843
<I/σ(I)>	20.12
Completeness [%]	100.0	100
Redundancy	35.5
CC(1/2)		0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M NaCl 0.15 M NH4SO4 0.1 M MES 18-22% PEG1000 2 mM AgNO3