6Z7Y

Human insulin in complex with the analytical antibody OXI-005 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	110.390, 173.230, 83.800
Unit cell angles	90.00, 135.83, 90.00

Refinement procedure

Resolution	48.420 - 2.200
R-factor	0.2182
Rwork	0.217
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z7x
RMSD bond length	0.009
RMSD bond angle	1.273
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3714)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.420	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.092	0.858
Rmeas	0.110	1.016
Rpim	0.059	0.540
Number of reflections	54923	19188
<I/σ(I)>	8.88	1.37
Completeness [%]	99.0	99.59
Redundancy	3.4	3.5
CC(1/2)	0.993	0.549

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.2 M magnesium chloride, 0.1 M Tris, pH 8, 20 % (w/v) PEG 6000