6Z7E
Variant Surface Glycoprotein VSGsur mutant H122A soaked in 7.7 mM suramin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-15 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 46.936, 71.033, 130.614 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.160 - 1.663 |
R-factor | 0.1857 |
Rwork | 0.184 |
R-free | 0.20830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6z79 |
Data scaling software | SCALA |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.160 | 1.723 |
High resolution limit [Å] | 1.663 | 1.663 |
Number of reflections | 52136 | 5028 |
<I/σ(I)> | 19.44 | 1.9 |
Completeness [%] | 99.8 | |
Redundancy | 13.2 | |
CC(1/2) | 1.000 | 0.559 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 19-24 % PEG 400 100 mM TEA/HCl pH=7.5 10 % (v/v) isopropanol |