6Z7D
Variant Surface Glycoprotein VSGsur mutant H122A soaked in 0.77 mM Suramin.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-15 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 46.900, 70.950, 130.443 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.080 - 1.750 |
R-factor | 0.1792 |
Rwork | 0.178 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6z79 |
Data scaling software | SCALA |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.080 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 44210 | 4304 |
<I/σ(I)> | 16.67 | |
Completeness [%] | 98.9 | 97.44 |
Redundancy | 13.3 | 13.8 |
CC(1/2) | 0.999 | 0.503 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 19-24 % PEG 400 100 mM TEA/HCl pH=7.5 10 % Isopropanol |