6Z6E

Crystal structure of the HK97 bacteriophage small terminase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	H 3
Unit cell lengths	123.281, 123.281, 78.991
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.230 - 1.400
R-factor	0.1596
Rwork	0.159
R-free	0.17040
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.443
Data reduction software	XDS (Oct 15, 2015)
Data scaling software	XDS (Oct 15, 2015)
Phasing software	SHELXD (2013/2)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.230	1.430
High resolution limit [Å]	1.400	1.400
Rmerge	0.049	0.707
Number of reflections	86871	3870
<I/σ(I)>	10.3	1.1
Completeness [%]	98.8
Redundancy	2.7
CC(1/2)	0.997	0.525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	9% (w/v) PEG 3350, 0.1 M succinic acid, pH 7.0