6Z67

FtsE structure of Streptococcus pneumoniae in complex with AMPPNP at 2.4 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	33.068, 117.901, 81.484
Unit cell angles	90.00, 98.11, 90.00

Refinement procedure

Resolution	47.596 - 2.400
R-factor	0.1919
Rwork	0.189
R-free	0.25230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ouk
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.600	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.100	0.550
Number of reflections	23969	2528
<I/σ(I)>	10.6
Completeness [%]	99.0
Redundancy	5.8
CC(1/2)	0.990	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.15 M NaF and 16% (w/v) PEG3350