6Z4E

The structure of the C-terminal domain of RssB from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-05-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	56.239, 56.239, 294.462
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.533 - 2.000
R-factor	0.2133
Rwork	0.212
R-free	0.24250
Structure solution method	MIR
RMSD bond length	0.003
RMSD bond angle	0.630
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.100
Number of reflections	18222	1408
<I/σ(I)>	25.2
Completeness [%]	91.8
Redundancy	20
CC(1/2)	1.000	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG1000