6Z4C

The structure of the N-terminal domain of RssB from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-05-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 32
Unit cell lengths	45.460, 45.460, 95.988
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.420 - 2.000
R-factor	0.20726
Rwork	0.220
R-free	0.26655
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pl1
RMSD bond length	0.009
RMSD bond angle	1.629
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.000	2.060
Rmerge	0.400
Number of reflections	13508	1298
<I/σ(I)>	8.1
Completeness [%]	96.7
Redundancy	2.2
CC(1/2)	0.400	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG1000