6Z46

Structure of the S. acidocaldarius 20S proteasome (Saci0613/Saci0662)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	292.15
Detector technology	PIXEL
Collection date	2014-06-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	108.287, 193.654, 323.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.190 - 3.698
R-factor	0.2897
Rwork	0.287
R-free	0.33440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j2q
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.190	3.830
High resolution limit [Å]	3.698	3.698
Number of reflections	73138	6972
<I/σ(I)>	5.64
Completeness [%]	99.2
Redundancy	1.9779
CC(1/2)	0.969	0.555

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292.15	0.1M MES pH 6, 6% PEG 6000, 0.1 M MgCl2