6Z42
The low resolution structure of a zinc-dependent alcohol dehydrogenase from Halomonas elongata.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 104.662, 104.662, 516.213 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.496 - 4.000 |
| R-factor | 0.3326 |
| Rwork | 0.329 |
| R-free | 0.36710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BALBES MODEL |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.540 | 4.470 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 3.990 | |
| Rpim | 0.150 | |
| Number of reflections | 15280 | 4182 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 30.9 | 32.9 |
| CC(1/2) | 0.994 | 0.643 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | B8 PACT SCREEN (Molecular Dimensions) |
