6Z3K

Structure of protective antibody 38-1-10A Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 2
Unit cell lengths	292.350, 87.130, 177.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.520 - 2.700
R-factor	0.1905
Rwork	0.188
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dgg
RMSD bond length	0.004
RMSD bond angle	0.745
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (dev_3699)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.500	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.370
Number of reflections	124934	6090
<I/σ(I)>	16.4	1.1
Completeness [%]	100.0
Redundancy	53.8
CC(1/2)	1.000	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	20% (v/v) PEG 6000, 0.1 M citrate, pH 4.0, 1 M lithium chloride