6Z3E
Crystal structure of GDP-bound human GIMAP5, amino acid residues 1-276
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-26 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.280, 57.230, 106.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.036 - 2.800 |
R-factor | 0.217666000947 |
Rwork | 0.215 |
R-free | 0.26815 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xtp |
RMSD bond length | 0.004 |
RMSD bond angle | 0.819 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.017 | 0.475 |
Number of reflections | 6579 | 595 |
<I/σ(I)> | 9.37 | |
Completeness [%] | 98.0 | |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 16% 2-propanol 50 mM ammonium acetate |