6Z3E

Crystal structure of GDP-bound human GIMAP5, amino acid residues 1-276

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-26
Detector	RAYONIX MX-225
Wavelength(s)	0.918
Spacegroup name	P 21 21 21
Unit cell lengths	41.280, 57.230, 106.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.036 - 2.800
R-factor	0.217666000947
Rwork	0.215
R-free	0.26815
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xtp
RMSD bond length	0.004
RMSD bond angle	0.819
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.017	0.475
Number of reflections	6579	595
<I/σ(I)>	9.37
Completeness [%]	98.0
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	16% 2-propanol 50 mM ammonium acetate