6Z31
Human cation-independent mannose 6-phosphate/ IGF2 receptor domain 8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-01-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.555, 45.537, 70.275 |
Unit cell angles | 90.00, 116.36, 90.00 |
Refinement procedure
Resolution | 36.020 - 2.560 |
Rwork | 0.223 |
R-free | 0.25080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Domain 10 from the crystal structure of cation-independent mannose 6-phosphate receptor domains 9-10 (separate deposition) |
Data reduction software | xia2 (0.5.902) |
Data scaling software | DIALS (1.14) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX ((1.18_3855: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.020 | 2.650 |
High resolution limit [Å] | 2.560 | 2.560 |
Number of reflections | 12121 | 1170 |
<I/σ(I)> | 5.59 | 1.97 |
Completeness [%] | 99.0 | 95.82 |
Redundancy | 4.7 | 4.6 |
CC(1/2) | 0.975 | 0.686 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 1.5 M Ammonium sulphate, 0.1 M Tris pH 8.5, 12 % glycerol |