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6Z31

Human cation-independent mannose 6-phosphate/ IGF2 receptor domain 8

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I24
Synchrotron siteDiamond
BeamlineI24
Temperature [K]80
Detector technologyPIXEL
Collection date2020-01-26
DetectorDECTRIS PILATUS3 6M
Wavelength(s)0.9686
Spacegroup nameP 1 21 1
Unit cell lengths65.555, 45.537, 70.275
Unit cell angles90.00, 116.36, 90.00
Refinement procedure
Resolution36.020 - 2.560
Rwork0.223
R-free0.25080
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)Domain 10 from the crystal structure of cation-independent mannose 6-phosphate receptor domains 9-10 (separate deposition)
Data reduction softwarexia2 (0.5.902)
Data scaling softwareDIALS (1.14)
Phasing softwarePHASER (2.8.3)
Refinement softwarePHENIX ((1.18_3855: ???))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]36.0202.650
High resolution limit [Å]2.5602.560
Number of reflections121211170
<I/σ(I)>5.591.97
Completeness [%]99.095.82
Redundancy4.74.6
CC(1/2)0.9750.686
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP8.52981.5 M Ammonium sulphate, 0.1 M Tris pH 8.5, 12 % glycerol

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