6Z31

Human cation-independent mannose 6-phosphate/ IGF2 receptor domain 8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-01-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 1 21 1
Unit cell lengths	65.555, 45.537, 70.275
Unit cell angles	90.00, 116.36, 90.00

Refinement procedure

Resolution	36.020 - 2.560
Rwork	0.223
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Domain 10 from the crystal structure of cation-independent mannose 6-phosphate receptor domains 9-10 (separate deposition)
Data reduction software	xia2 (0.5.902)
Data scaling software	DIALS (1.14)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX ((1.18_3855: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.020	2.650
High resolution limit [Å]	2.560	2.560
Number of reflections	12121	1170
<I/σ(I)>	5.59	1.97
Completeness [%]	99.0	95.82
Redundancy	4.7	4.6
CC(1/2)	0.975	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	1.5 M Ammonium sulphate, 0.1 M Tris pH 8.5, 12 % glycerol