6Z2S

Crystal structure of adhibin analogue-bound myosin-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979384
Spacegroup name	C 2 2 21
Unit cell lengths	90.931, 144.220, 156.937
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.470 - 3.200
R-factor	0.2199
Rwork	0.218
R-free	0.26210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vom
RMSD bond length	0.006
RMSD bond angle	0.716
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.470	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	17402	1711
<I/σ(I)>	9.51
Completeness [%]	99.9
Redundancy	12.1
CC(1/2)	0.996	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	0.2 M lithium acetate and 20 % PEG 3350