6Z27

Photosynthetic Reaction Center From Rhodobacter Sphaeroides strain RV LCP crystallization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.8610
Spacegroup name	P 42 21 2
Unit cell lengths	99.914, 99.914, 234.047
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.860 - 2.100
R-factor	0.2092
Rwork	0.209
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v3y
RMSD bond length	0.008
RMSD bond angle	1.031
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (1.18_3861)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.130
High resolution limit [Å]	2.070	2.070
Rmerge	0.075
Number of reflections	72564	5862
<I/σ(I)>	15.94	1.14
Completeness [%]	99.9
Redundancy	11.06
CC(1/2)	0.990	0.310

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		289	1-mono-oleyl-rac-glycerol (MO), 1,2,3-heptanetriol, Jeffamine M600, N,N-dimethyldodecylamine-N-oxide (LDAO)