6Z26

Structure of the Danio rerio SAS-6 coiled-coil domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-16
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97930
Spacegroup name	P 21 21 21
Unit cell lengths	69.380, 70.306, 98.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.270 - 2.320
R-factor	0.278
Rwork	0.277
R-free	0.29700
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.190
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.270	2.370
High resolution limit [Å]	2.310	2.310
Rmerge	0.121	0.599
Rmeas	0.127	0.624
Number of reflections	14143	840
<I/σ(I)>	9.9	3.5
Completeness [%]	65.1	4.2
Redundancy	13	12.7
CC(1/2)	0.990	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	4 mM of alkalis mixture (barium acetate, caesium acetate, rubidium chloride, strontium acetate) 0.1 M BES / Triehylamine buffer 50% v/v of a precipitant mix (25% w/v PEG 4000 and 40% w/v 1,2,6- Hexanetriol)