6Z1X
Crystal structure of human steroid carrier protein SL (SCP-2L) mutant V83C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-13 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.690, 48.850, 63.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.820 - 2.090 |
| R-factor | 0.1833 |
| Rwork | 0.181 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ikt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.574 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.820 | 2.130 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 6538 | 319 |
| <I/σ(I)> | 23.4 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 277 | 2.2 M ammonium sulfate, 0.2 M NaCl and 0.1 M citric acid |
