6Z1W
Crystal structure of human steroid carrier protein SL (SCP-2L) mutant A100C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-16 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.375, 50.746, 63.096 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.040 - 2.480 |
R-factor | 0.2109 |
Rwork | 0.208 |
R-free | 0.26780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ikt |
RMSD bond length | 0.009 |
RMSD bond angle | 1.548 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.520 |
High resolution limit [Å] | 2.480 | 2.480 |
Number of reflections | 3729 | 217 |
<I/σ(I)> | 11.2 | |
Completeness [%] | 85.9 | |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 277 | 2.2 M ammonium sulfate, 0.2 M NaCl and 0.1 M citric acid |