6Z1E

Crystal structure of the AAA domain of Rubisco Activase from Nostoc sp. (strain PCC 7120)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.13956
Spacegroup name	H 3 2
Unit cell lengths	112.920, 112.920, 284.327
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.749 - 2.454
R-factor	0.215
Rwork	0.212
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z1d
RMSD bond length	0.005
RMSD bond angle	0.992
Data reduction software	XDS (VERSION September 26, 2012)
Data scaling software	SCALA (3.3.16)
Phasing software	MOLREP (10.2.35)
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.388	47.388	2.590
High resolution limit [Å]	2.454	7.760	2.454
Rmerge		0.012	1.085
Rmeas	0.042	0.013	1.182
Rpim	0.016	0.005	0.462
Total number of observations	171034
Number of reflections	25855	900	3668
<I/σ(I)>	29.3	46.3	0.7
Completeness [%]	99.8	99.4	98.6
Redundancy	6.6	6.4	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	277	2.2 M Na-acetate