6Z1D

Crystal structure of the AAA domain of Rubisco Activase from Nostoc sp. (strain PCC 7120), Gadolinium complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.68745
Spacegroup name	H 3 2
Unit cell lengths	111.990, 111.990, 283.818
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.633 - 2.705
R-factor	0.2179
Rwork	0.216
R-free	0.25850
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.098
Data reduction software	XDS (VERSION September 26, 2012)
Data scaling software	SCALA (3.3.16)
Phasing software	SHELXDE (Version 2006/3)
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.990	48.990	2.850
High resolution limit [Å]	2.705	8.550	2.705
Rmerge		0.017	0.624
Rmeas	0.069	0.022	0.658
Rpim	0.015	0.005	0.148
Total number of observations	375979
Number of reflections	19125	674	2738
<I/σ(I)>	37.2	35	1.3
Completeness [%]	99.9	99.6	99.5
Redundancy	19.7	19.3	19.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	277	2.2 M Na-acetate