6Z1C
Crystal structure of Arabidopsis thaliana CK2-alpha-1 in complex with TTP-22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-10 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9801 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 85.250, 81.110, 48.380 |
Unit cell angles | 90.00, 108.64, 90.00 |
Refinement procedure
Resolution | 45.840 - 1.750 |
R-factor | 0.1968 |
Rwork | 0.195 |
R-free | 0.23830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xx6 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.873 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.842 | 5.220 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 31142 | 31165 |
<I/σ(I)> | 5 | |
Completeness [%] | 98.8 | |
Redundancy | 1.1 | |
CC(1/2) | 0.991 | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M sodium sulfate 0.1M Bis-Tris propane pH 6.5 20% PEG 3350 |