6Z1C

Crystal structure of Arabidopsis thaliana CK2-alpha-1 in complex with TTP-22

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	C 1 2 1
Unit cell lengths	85.250, 81.110, 48.380
Unit cell angles	90.00, 108.64, 90.00

Refinement procedure

Resolution	45.840 - 1.750
R-factor	0.1968
Rwork	0.195
R-free	0.23830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xx6
RMSD bond length	0.007
RMSD bond angle	0.873
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.842	5.220
High resolution limit [Å]	1.750	1.750
Number of reflections	31142	31165
<I/σ(I)>	5
Completeness [%]	98.8
Redundancy	1.1
CC(1/2)	0.991	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M sodium sulfate 0.1M Bis-Tris propane pH 6.5 20% PEG 3350