6Z0W

Crystal structure of the cytoplasmic domain of FlhB from Shewanella putrefaciens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976244
Spacegroup name	H 3 2
Unit cell lengths	152.436, 152.436, 126.886
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.440 - 2.100
R-factor	0.212
Rwork	0.210
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b0z
RMSD bond length	0.011
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.440	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.141	1.233
Number of reflections	33095	3286
<I/σ(I)>	13.46	1.64
Completeness [%]	100.0
Redundancy	19.9
CC(1/2)	0.999	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	XX