6Z0K
Crystal structure of laccase from Pediococcus acidilactici Pp5930 (Hepes pH 7.5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-02 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97927 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.070, 78.188, 162.493 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.560 - 2.000 |
R-factor | 0.1979 |
Rwork | 0.197 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zdw |
RMSD bond length | 0.004 |
RMSD bond angle | 1.228 |
Data reduction software | XDS |
Data scaling software | Aimless (0.2.8) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 162.490 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.090 | 0.668 |
Rmeas | 0.097 | 0.727 |
Rpim | 0.038 | 0.285 |
Total number of observations | 304133 | |
Number of reflections | 45687 | 3457 |
<I/σ(I)> | 11.9 | |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 6.7 | 6.3 |
CC(1/2) | 0.992 | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 10% PEG8000 10% ethylene glycol 0.1 M HEPES pH 7.5 |