6Z0J
Crystal structure of laccase from Pediococcus acidilactici Pa5930 (Tris-HCl pH 8.5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-06 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97925 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.797, 94.294, 97.564 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.800 - 2.500 |
R-factor | 0.2227 |
Rwork | 0.220 |
R-free | 0.26790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zdw |
RMSD bond length | 0.003 |
RMSD bond angle | 1.264 |
Data reduction software | XDS |
Data scaling software | Aimless (0.2.8) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 94.290 | 94.290 | 2.600 |
High resolution limit [Å] | 2.500 | 9.010 | 2.500 |
Rmerge | 0.148 | 0.063 | 0.904 |
Rmeas | 0.160 | 0.069 | 0.970 |
Rpim | 0.058 | 0.027 | 0.350 |
Total number of observations | 124640 | 2648 | 14100 |
Number of reflections | 17180 | 437 | 1879 |
<I/σ(I)> | 7.9 | 17.3 | 2.2 |
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 7.3 | 6.1 | 7.5 |
CC(1/2) | 0.995 | 0.995 | 0.758 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 30% PEG1000 and 0.1M Tris-HCl pH 8.5 |