6Z0D

tRNA-Guanine Transglycosylase (TGT) H333F mutant crystallised at pH 5.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER IMUS MICROFOCUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-05-07
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	90.791, 65.332, 70.574
Unit cell angles	90.00, 96.30, 90.00

Refinement procedure

Resolution	40.000 - 1.650
R-factor	0.1654
Rwork	0.164
R-free	0.19440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pud
RMSD bond length	0.006
RMSD bond angle	0.775
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.750
High resolution limit [Å]	1.650	1.650
Number of reflections	47815	6664
<I/σ(I)>	22.25
Completeness [%]	96.7
Redundancy	3.6
CC(1/2)	0.999	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	288	100 mM MES pH 5.5, 0.5 mM DTT, 10% (v/v) DMSO, 13% (m/v) PEG8000