6Z06
Crystal structure of Puumala virus Gc in complex with Fab 4G2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-23 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.968590 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 97.213, 50.449, 124.446 |
Unit cell angles | 90.00, 107.66, 90.00 |
Refinement procedure
Resolution | 92.633 - 3.500 |
R-factor | 0.2196 |
Rwork | 0.217 |
R-free | 0.26390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5j81 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX (v1.14) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 92.633 | 92.633 | 3.550 |
High resolution limit [Å] | 3.500 | 9.470 | 3.500 |
Rmeas | 0.437 | 0.173 | 1.489 |
Rpim | 0.140 | 0.057 | 0.511 |
Number of reflections | 14920 | 818 | 702 |
<I/σ(I)> | 4.6 | 8.8 | 1.5 |
Completeness [%] | 99.8 | 100 | 93 |
Redundancy | 9.6 | 8.8 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 20% PEG6000, 1 M LiCl, 0.1 M MES pH 6, 6% 2-methyl-2,4-pentanediol |