6Z03
DNA Topoisomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-08 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.470, 94.680, 92.650 |
| Unit cell angles | 90.00, 111.81, 90.00 |
Refinement procedure
| Resolution | 70.070 - 2.200 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.24130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6z01 |
| Data reduction software | XDS (Jan 26, 2018) |
| Data scaling software | autoPROC (1.1.7 (20171219)) |
| Phasing software | MOLREP (CCP4Interface 7.0.053) |
| Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.016 | 2.235 |
| High resolution limit [Å] | 2.197 | 2.197 |
| Rmerge | 0.982 | |
| Number of reflections | 61691 | 3039 |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.998 | 0.668 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 22% PEG 6000, 1 M LiCl 100mM Sodium Acetate |
